Heart failure & cardiomyopathies
A Pilot Study on DNA Methylation Changes for Non-Invasive Molecular Diagnostics in Heart Failure
Authors' conclusion
We demonstrate that circulating CD4+ T cell-specific methylation levels of network-oriented CKAP4, CPT1A, EIF2S2, SPTB, SYT6 and RAB11FIP1 genes can distinguish between IsHF and NIsHF. In particular, hypomethylation of EIF2S2, SPTB and RAB11FIP1 genes is significantly correlated with key clinical features in isHF patients
Study context
- Population
- Patients with HF with reduced ejection fraction (HFrEF); DNA methylation in circulating CD4+ T lymphocytes (reduced representation bisulfite sequencing (RRBS))
- Participants
- 8 vs 4
- Comparison
- IsHF vs NIsHF
- IsHFn = 8
- NIsHFn = 4reference
- Primary endpoint
- Differentially methylated CpG positions distinguishing IsHF from NIsHF
Methodological detailspilot study, network-oriented analysis
- Pilot study
- Network-oriented analysis
Primary result
As reported in the abstract
Network-oriented DMPs distinguishing IsHF vs NIsHF
- 6 DMPs distinguished IsHF vs NIsHF
- AUC > 0.8
- DMPs significantly hypomethylated in IsHF vs NIsHF, annotated to CKAP4, CPT1A, EIF2S2, spectrin beta (SPTB), SYT6 and RAB11FIP1
- P < 0.05
Further findings
Correlation of CD4+ T cell methylation with clinical features (IsHF)
- EIF2S2 hypomethylation vs VO2 max
- ρ = 0.73, P = 0.04
- RAB11FIP1 hypomethylation vs MECKI score
- ρ = −0.85, P = 0.001
- SPTB vs VO2 max
- ρ = 0.73, P = 0.04
- SPTB vs LV mass index
- ρ = −0.91, P = 0.005
- SPTB vs LVEF
- ρ = 0.83, P = 0.01
Original publication
A Pilot Study on DNA Methylation Changes for Non-Invasive Molecular Diagnostics in Heart Failure. ESC Heart Fail. Published 2 September 2025. doi:10.
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