Heart failure & cardiomyopathies
Single-Cell Splicing Isoform Atlas of the Adult Human Heart and Heart Failure
Authors' conclusion
We present a comprehensive atlas of splicing isoforms in the normal adult heart and heart failure through long-read single-nucleus RNA sequencing and computational analyses. The results suggest crucial roles of isoforms in buffering core cellular programs and contributing to disease-associated cell states.
Study context
- Population
- Adult human left ventricle, normal and HF
- Comparison
- Normal adult LV vs HF LV
- Outcome
- Isoform heterogeneity and usage
Methodological detailslong-read single-nucleus RNA sequencing, Validation by RT-qPCR and targeted amplicon sequencing, Interactive web server of results
- Long-read single-nucleus RNA sequencing
- Validation by RT-qPCR and targeted amplicon sequencing
- Interactive web server of results
Main finding
As reported in the abstract
Cell type-specific genes in healthy LV that were polyform
≈30%
Ubiquitously expressed genes with cell type-specific differential isoform usage in normal hearts
>300
Genes with marked isoform usage shifts in cardiomyocytes across conditions, most predicted to change protein coding outcomes
379
Original publication
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