Heart failure & cardiomyopathies
Spatial transcriptional landscape of human heart failure
Authors' conclusion
These findings highlight the critical role of cell-enriched and histology-specific transcriptome mapping in understanding the complex pathophysiological landscape of failing hearts, offering molecular insights and potential therapeutic targets for future interventions.
Study context
- Population
- Human ventricular myocardium from various forms of cardiomyopathy and control samples90 tissue cores
- Participants
- 44 participants
- Outcome
- Cell-type- and histology-specific gene expression in cardiomyopathy
Methodological detailsGeoMx Whole Human Transcriptome Atlas; integrated with cell type, clinical and histological data; multiplex immunohistochemistry and prior sc/snRNA-seq data
- GeoMx Whole Human Transcriptome Atlas; integrated with cell type, clinical and histological data; multiplex immunohistochemistry and prior sc/snRNA-seq data
Main finding
As reported in the abstract
- GeoMx compartmentalization: high accuracy for cardiomyocytes, limitations for endothelial cells and fibroblasts
- Cardiomyocyte UCHL1 associated with degeneration
- Endothelial CCL14, ACKR1 and PLVAP linked to fibrosis
- PLVAP, ACKR1 and CCL14-positive pro-inflammatory endothelial cell subtype linked to fibrosis in heart failure (HF)
- Cardiomyocyte ribosomal protein downregulation associated with myocyte disarray in hypertrophic cardiomyopathy
- Pronounced inflammatory responses in end-stage HF
- CRIP3, PFKFB2 and TAX1BP3 identified as novel contributors to HF pathogenesis
Original publication
Spatial transcriptional landscape of human heart failure. Eur Heart J. Published 8 May 2025. doi:10.
Composed by AI from the published abstract and checked against it. How Briefs are made →