Transcriptional dynamics of pluripotent stem cell-derived endothelial cell differentiation revealed by single-cell RNA sequencing
Authors' conclusion
A transcriptional bifurcation into endothelial and mesenchymal lineages was identified, as well as novel transcriptional signatures underpinning commitment and maturation. The transcriptional architecture of hESC-ECP was distinct from mature and foetal human EC.
Study context
- Population
- Human embryonic stem cell-derived endothelial cells (hESC-EC) during directed 8-day differentiation
- Participants
- 105,727 cells
- Comparison
- hESC-derived endothelial cells vs mature endothelial cells from diverse vascular beds
- Outcome
- Transcriptional landscape of hESC-EC differentiation
Methodological detailssingle-cell RNA sequencing
- Single-cell RNA sequencing
Main finding
As reported in the abstract
hESC-ECP cells co-expressing CD31 and CD144
66%
Original publication
Transcriptional dynamics of pluripotent stem cell-derived endothelial cell differentiation revealed by single-cell RNA sequencing. Eur Heart J. Published 26 June 2019. doi:10.
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